Andrology Laboratory Masters

Andrology Laboratory Masters is organized by MasterHealthPro (MHP).

• Self-paced
• 6 months course
• Hours of effort - 2.5 hours/ week

Description:
This master’s andrology laboratory encompasses both the laboratory aspects and its clinical application in a detailed format for a comprehensive understanding of the subject of a pellucid reporting system. This course helps evaluate semen and its properties and elaborate on semen preparation for various ART procedures. The module delivered by experts includes lessons on all laboratory and Andrology protocols including Semen Analysis, Sperm Preparation Techniques, Semen Freezing, Advanced Sperm Function tests (Fructose, HOS, Vitality, Peroxidase Staining), Sperm DNA Fragmentation, Semen Banking & Sperm Retrieval Techniques. The module involves an ample number of video sessions for the audience to familiarize themselves with laboratory routines.

Learning Objectives:

• Understanding the workup for male infertility, including the importance of the physical examination
• Semen analysis and its Role in clinical Diagnosis and decision-making
• Restate the limitations of the primary semen analysis and the importance of advanced semen analysis
• Learn semen analysis and ancillary tests by the WHO sixth edition criteria
• Learn advanced sperm preparation methods used in assisted reproduction such as IUI, IVF, and ICSI
• Understand advanced sperm function tests
• Learn methods for male fertility preservation for use in ART

Learning Outcomes:

• Theoretical and technical knowledge of clinical Andrology
• Knowledge of setting up an Andrology lab
• Operational and Quality Management skills

Course Content:
Physiology & Anatomy

• Anatomy of the male reproductive system
• Sperm Transport and Maturation
• Seminal Plasma and Its Role
• Spermatogenesis and Spermiogenesis

Setting up of IUI Lab

• Setting Up of Andrology Laboratory
  • 1. Requirements
  • 2. Guidelines to setup IUI Lab

Lab Maintenance

• Quality Analysis and Quality Check
  • 1. Media and Disposables Handling
  • 2. Handling of Liquid Nitrogen
  • 3. LN2 Tank Maintenance
  • 4. Handling of Sero Positive Samples

Semen Analysis

• Macro Evaluation, Micro Analysis & DFI by Flow Cytrometry
• Semen Analysis: Practical aspects
• Interpretation Of Abnormal Semen Analysis
• WHO 5th & 6th Manual - Differences and Clinical Implications

Sperm Processing Techniques

• Sperm Preparation Techniques and Practical Implications
  • 1. Simple Wash
  • 2. Swim Up
  • 3. Double Density Gradient
  • 4. Microfluidics
  • 5. Retrograde Ejaculation Sample Preparation
  • 6. TESA Sperm processing Technique

Sperm Freezing Techniques

• Sperm Cryopreservation and Sperm Vitrification with fundamentals of cryobiology and implications to practice
  • 1. Slow Freezing Methods
  • 2. Vitrification
  • 3. TESA Sample Freezing

Upskilling

• CASA
• MACS & U Select Method