Embryology Laboratory Fellowship

Embryology Laboratory Fellowship is organized by MasterHealthPro (MHP).

• Self-paced
• 3 months course
• Hours of effort - 2.5 hours/ week

Description:

This program is the first of its kind designed for embryologists/clinicians involved in Assisted Reproductive Technology (ART) practice where you will learn the basics of procedures and protocols practiced in an IVF laboratory. The module delivered by experts includes lessons on setting up a new lab, acquiring equipment, quality control management, troubleshooting performance analysis, etc. The module involves an ample number of video sessions for the audience to familiarize themselves with laboratory routines. The program is designed by internationally acclaimed experts in the field of ART and state-of-the-art lectures are delivered by world-renowned faculties from across the globe.

Learning Objectives:

• To learn about the cell biology and development of gametes and embryos
• Understand the fundamentals and principles of procedures involved in IVF
• Learn how to set up an IVF laboratory
• To impart theoretical and practical knowledge of the skills and techniques used in the micromanipulation of gametes
• Learn about various clinical aspects of assisted reproduction techniques

Learning Outcomes:

• Theoretical and technical knowledge of the clinical embryology
• Clinical methodologies in IVF (In vitro Fertilization) and advanced developments
• Operational and Quality Management skills in an IVF laboratory
• Troubleshooting and crisis management

Course Content:

Cell Biology of Embryology

• Oogenesis, Spermatogenesis & Embryogenesis

Setting Up an ART Lab

• Setting up of ART Lab
• 1. Clean Air System
• 2. Incubators
• 3. Laminar Air Flow System
• 4. Microscopes
• 5. Micromanupulators
• 6. Other essential Equipments

Introduction to IVF and ICSI Procedures

• Media & Disposables
• 1. Contents of Media
• 2. Handling of Media
• 3. Types of Disposables
• 4. Usage

Preparing Lab for the ART Procedures

• Preparing Lab for the ART Procedures
• 1. Particulate Check
• 2. VOC level Check
• 3. pH Check
• 4. Temperature Check
• 5. CO2 Check
• 6. Daily Check List
• 7. Monthly Check List
• 8. Annual check List

Sperm Preparation Techniques for IVF and ICSI Procedures

• Sperm Preparation Techniques and Practical Implications
• 1. Simple Wash
• 2. Density Gradient
• 3. Swim Up Technique
• 4. Retrograde Ejaculate Sample Processing
• 5. Testicular & Epididymal Sperm Processing Techniques

ART Procedures and Preparation

• Ovum Pick Up
• Conventional IVF
• Denudation
• Dish & Media Preparation
• ICSI & Oocyte Morphology
• Fertilization Check and Embryo Grading
• Embryo Transfer

Cryopreservation Procedures

• Oocyte & Embryo vitrification & Embryo Warming

Trouble Shooting in IVF Lab

• Trouble Shooting in IVF Lab

Documentation according guidelines

• Documentation according guidelines

Upgrading of Skills

• Upgrading of skills
• 1. Timelapse technology
• 2. IMSI
• 3. PICSI
• 4. Embryo Biopsy
• 5. Testicular Sperm Selection
• 6. Laser Assisted Hatching
• 7. Artificial activation of Oocyte

Lab Maintenance

• Quality Analysis and Quality Check
• 1. Media and Disposables Handling
• 2. Handling of Liquid Nitrogen
• 3. LN2 Tank Maintenance
• 4. Handling of Sero Positive Samples

Upskilling

• CASA
• DFI by Flow Cytrometry
• MACS & U Select Method