Embryology Laboratory Masters

Embryology Laboratory Masters is organized by MasterHealthPro (MHP).

• Self-paced
• 6 months course
• Hours of effort - 2.5 hours/ week

Description:
This is the first of its kind elaborate program for embryologists and clinicians involved in Assisted Reproductive Technology (ART) practice where you will learn the basics, advances, and research development of procedures and protocols practiced in an IVF laboratory. The module delivered by experts includes lessons on setting up a new lab, acquiring equipment, quality control management, troubleshooting and performance analysis, etc. The specialty of this carefully constructed master's course is giving an embryological outlook from a clinician's perspective for a better understanding between embryologists and clinicians for successful ART outcomes. The module involves an ample number of video sessions for the audience to familiarize themselves with laboratory routines. The program is designed by internationally acclaimed experts in the field of ART and state-of-the-art lectures are delivered by world-renowned faculties from across the globe.

Learning Objectives:

• To learn about the cell biology and development of gametes and embryos
• Understand the fundamentals and principles of procedures involved in IVF
• Learn how to set up an IVF laboratory
• To impart theoretical and practical knowledge of the skills and techniques used in the micromanipulation of gametes
• Learn about various clinical aspects of assisted reproduction techniques
• Understanding the workup for male infertility, including the importance of the physical examination
• Semen analysis and its Role in clinical Diagnosis and decision making
• Restate the limitations of the primary semen analysis and the importance of advanced semen analysis and its importance in the embryology lab

Learning Outcomes:

• Theoretical and technical knowledge of clinical embryology
• Clinical methodologies in IVF (In-vitro Fertilization) and advanced developments
• Operational and Quality Management skills in an IVF laboratory
• Troubleshooting and crisis management

Course Content:
Cell Biology of Embryology

• Anatomy of the male reproductive system
• Anatomy of the female reproductive system
• Oogenesis, Spermatogenesis & Embryogenesis

Setting Up an ART Lab

• Setting up of ART Lab
  • 1. Clean Air System
  • 2. Incubators
  • 3. Laminar Air Flow System
  • 4. Microscopes
  • 5. Micromanupulators
  • 6. Other essential Equipments

Introduction to IVF and ICSI Procedures

• Media & Disposables
  • 1. Contents of Media
  • 2. Handling of Media
  • 3. Types of Disposables
  • 4. Usage

Preparing Lab for the ART Procedures

• Preparing Lab for the ART Procedures
  • 1. Particulate Check
  • 2. VOC level Check
  • 3. pH Check
  • 4. Temperature Check
  • 5. CO2 Check
  • 6. Daily Check List
  • 7. Monthly Check List
  • 8. Annual check List

Sperm Preparation Techniques for IVF and ICSI Procedures

• Sperm Preparation Techniques and Practical Implications
  • 1. Simple Wash
  • 2. Density Gradient
  • 3. Swim Up Technique
  • 4. Retrograde Ejaculate Sample Processing
  • 5. Testicular & Epididymal Sperm Processing Techniques

ART Procedures and Preparation

• Ovum Pick Up
• Conventional IVF
• Denudation
• Dish & Media Preparation
• ICSI & Oocyte Morphology
• Fertilization Check and Embryo Grading
• Embryo Transfer

Cryopreservation Procedures

• Oocyte & Embryo vitrification & Embryo Warming

Trouble Shooting in IVF Lab

• Trouble Shooting in IVF Lab

Documentation according guidelines

• Documentation according guidelines

Upgrading of Skills

• Upgrading of skills
  • 1. Timelapse technology
  • 2. IMSI
  • 3. PICSI
  • 4. Embryo Biopsy
  • 5. Testicular Sperm Selection
  • 6. Laser Assisted Hatching
  • 7. Artificial activation of Oocyte

Lab Maintenance

• Quality Analysis and Quality Check
  • 1. Media and Disposables Handling
  • 2. Handling of Liquid Nitrogen
  • 3. LN2 Tank Maintenance
  • 4. Handling of Sero Positive Samples

Upskilling

• CASA
• DFI by Flow Cytrometry
• MACS & U Select Method

Semen Analysis and practical utility

• Semen Analysis: Practical aspects
• Interpretation Of Abnormal Semen Analysis
• WHO 5th & 6th Manual - Differences and Clinical Implications

Advanced Sperm Function Tests and Techniques

• Male factor in recurrent pregnancy loss

Infertility management In Special Situations

• Treatment of HIV positive males and conception
• Management of Anejaculation & Erection and Ejaculatory Dysfunction Relevant to Fertility

Azoospermia Evaluation and Management

• Sperm retrieval techniques: Critical answers for decision making

Optimising Male Infertility Practice

• Overview of IUI vs IVF vs ICSI and guidelines for clinical practice