OVERVIEW

White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions is organized by MediaLab, Inc.

Course Overview:
This collection of informative case studies covers current topics in white cell and platelet disorders. Topics include leukemoid reactions, leukocytosis, intracellular granules, intra-cytoplasmic inclusion bodies, platelet satellitism, and more. Excellent for continuing education for clinical laboratory science professionals.

Objectives:
• Recognize several peripheral white cell abnormalities and list the criteria necessary to request a morphological/clinical review.
• Recognize and list the criteria by which a leukemoid reaction is differentiated from leukocytosis with a left shift.
• Describe various intracellular white blood cell inclusions and correlate them with the metabolic, genetic, and reactive conditions with which they are associated.
• Estimate platelet counts from peripheral blood smear scans and discuss erroneous automated platelet results.

Course Outline:
• White Blood Cell Disorders and Platelets
• Peripheral Smear Review Introduction
• Peripheral Blood Smear Preparation
• Evaluation Criteria: White Blood Cells and Platelets
• Criteria for Performing a Manual Review of the Smear
• Which of the images below represents an area of appropriate cell density to choose for performance of a manual differential?
• Platelet Estimates
• An automated platelet count of 40.0 X 109/L was reported. Review of the peripheral blood smear (see image to the right) reveals single platelets in op...
• Additional Comments
• Leukemoid Reaction and Toxic Changes
• Leukemoid Reaction
• Toxic Changes
• Thrombocytosis Associated with Toxic Granulation
• Case Study One
• The neutrophils illustrated in this image are representative of those seen in a female patient's peripheral blood smear. The total WBC was 28.5 X 109/...
• Toxic granulation noted in the neutrophils' cytoplasm reflects an increase in activity of which of the following? (Choose all that apply)
• A peripheral blood smear with many myeloid cells was presented for morphology review (see image on the right). Toxic granulation and vacuoles in the n...
• The association of increased platelets accompanying neutrophilia and toxic granulation, as illustrated in this image, is called thrombocytopenia.
• White Cell Nuclear and Cytoplasmic Abnormalities Related to Inherited Conditions
• Pelger-Huet Anomaly
• Chediak-Higashi Anomaly
• Alder Anomaly
• May-Hegglin Anomaly
• The inclusions noted in the cytoplasm of this white blood cell are most suggestive of which of these conditions?
• WBC inclusions: Summary
• Familial disorders: summary
• Case Study Two:The patient is a 10 year-old boy from Florida who developed abdominal pain while on a skiing trip with his family in Colorado. He was b...
• Case Study Two: Follow-up
• The cell indicated by the arrow in the image on the right is most consistent with Chediak-Higashi anomaly.
• Miscellaneous Granulocytic Cells
• Eosinophils
• Basophils
• A peripheral blood smear was reviewed and a representative field is shown on the right. Which of the following conditions may produce the results seen...
• Which of the following conditions is NOT associated with an increase in the white blood cell shown in the image on the right?
• Non Granulocytic White Blood Cells
• Disorders and Diseases Associated With Increased Monocytes
• Case Study Three
• Case Study Four
• Case Study Five
• Multiple Myeloma
• Smudge Cells
• Platelets
• Giant Platelets
• Thrombocytopenia
• Pseudo-thrombocytopenia
• Thrombocytosis
• Platelet satellites (see image to the right) may account for low platelet counts as determined by automated hematology cell counters. Satellitosis is ...
• The image on the right is a microscopic field from a Wright-Giemsa stained peripheral blood smear (1000X magnification). The structure indicated by th...
• A representative field from a peripheral blood smear, seen on the right, was held for morphological and clinical review as the total platelet count wa...
• The peripheral blood smear shown in this image was held for review because of an increased platelet count. Conditions in which platelets are increased...
• This image is representative of a peripheral blood smear. As a result of this phenomenon, some automated hematology analyzers may report:
• References
• References