Chemical Screening of Urine by Reagent Strip

Chemical Screening of Urine by Reagent Strip is organized by MediaLab, Inc.

Course Description:
This course discusses the chemical tests that are an integral part of a urinalysis. Proper specimen handling and technqiue are discussed for key tests, including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, and leukocyte esterase.

Objectives:
• Define the optimal storage time and temperature for urinalysis samples.
• Review the proper storage of and procedure for the use of urinalysis reagent strips.
• Describe the chemical reactions, quality control measures, and interpretation of results for urinalysis reagent strip analytes including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, leukocyte esterase.
• Explain the proper techniques used during urinalysis reagent strip testing; ensuring accuracy.
• Identify conditions and/or substances that may interfere with urinalysis reagent strip testing.
• Identify the additional testing protocols used to confirm urinalysis reagent strip testing results.
• Summarize the analytical principles of measurement in urinalysis reagent strip instruments.

Course Outline:
• Introduction to Urine Chemical Reagent Strips
    • Chemical Urinalysis Reagent Strips
    • Urine Specimen Collection
    • Urine Specimen Transport
    • Reagent Strip Procedure: Manual or Semi-Automated Methods
    • Handling and Storage of Urine Reagent Strips
    • Which of the following statements is true regarding the storage and handling of urine reagent strips?
    • All the following statements about the urine specimen are true except:
    • Which of the following statements is true for the reagent strip procedure?
    • Quality control procedures should be performed with each new lot of chemical reagent strips and as often as required by the laboratory's procedure.
• Urine pH Analysis
    • pH Analysis: The Urine Specimen
    • pH Reaction
    • Urine pH: Acidic and Alkaline
    • A urine specimen was collected at 6:00 AM and remained at room temperature until it was received in the laboratory at 3:30 PM. How may the pH of the s...
    • Match the following factors with the expected urine pH type.
• Urine Specific Gravity Analysis
    • Measuring Specific Gravity (SG)
    • Advantages and Limitations of the Chemical Reagent Strip Method for Specific Gravity (SG)
    • Clinical Significance of Specific Gravity
    • How does ion concentration in the urine relate to specific gravity?
• Urine Protein Analysis
    • Protein Error of Indicators
    • Confirmatory Testing for Urine Protein
    • Clinical Significance of Urine Protein
    • Clinical Significance of Urine Protein, continued
    • Follow-up Testing for Positive Urine Protein
    • The presence of increased levels of protein in the urine may be an early indicator of which of the following conditions?
    • Examples of conditions resulting in benign proteinuria include: (Choose ALL correct answers)
• Urine Glucose Analysis
    • Glucose Reaction
    • False-Positive and False-Negative Urine Glucose Results
    • Clinical Significance of Glucose in the Urine
    • Which of the following conditions produce glycosuria (glucose in the urine)? Choose all that apply.
• Urine Ketone Analysis
    • Ketones Overview
    • Ketone Reaction
    • False-Positive and False-Negative Ketone Test Results
    • Clinical Significance of Urine Ketones
    • The ketone component that is measured by the nitroprusside reaction is:
    • Which of the following can cause a false-positive result for ketones?
    • Significantly increased levels of ketones are detected in the urine with which of the following conditions? Choose all that apply.
• Urine Blood Analysis
    • Introduction to Hematuria
    • Blood Reaction
    • False-Positive and False-Negative Results
    • Clinical Significance of Blood in Urine
    • A urine sample is cloudy pink in appearance. The microscopic examination reveals the presence of intact red blood cells (RBCs). What is the term that ...
    • Which of the following substances can cause a false-positive result for blood on the urine reagent strip?
• Urine Nitrite Analysis
    • Nitrite
    • Nitrite Reaction
    • Nitrite Test Sensitivity
    • False-Positive and False-Negative Results
    • Clinical Significance of Nitrite in Urine
    • Nitrate in urine is reduced to nitrite by:
• Urine Leukocytes
    • Leukocytes Reaction
    • Leukocytes Reaction, continued
    • False-Positive and Falsely-Decreased Results
    • Clinical Significance of Leukocytes in Urine
    • To screen for urinary tract infection, the leukocytes test result should be evaluated along with the results from which of these other reagent strip t...
• Urine Bilirubin Analysis
    • Overview of Bilirubin
    • Bilirubin Reaction
    • False-Positive and False-Negative Urine Bilirubin Results
    • Ruling out False-Positive Urine Bilirubin Test Results
    • Clinical Significance of Urine Bilirubin
    • Which of the following may cause a false positive bilirubin result on a urine chemical reagent strip?
    • When a patient has a bile duct obstruction, the bilirubin test portion of the reagent strip is:
    • A positive reagent strip bilirubin test indicates the presence of what form of bilirubin in the urine?
• Urine Urobilinogen Analysis
    • Introduction to Urobilinogen
    • Urobilinogen Reaction
    • False-Positive and False-Negative Urobilinogen Results
    • Clinical Significance of Urobilinogen in Urine
• Automation of Chemical Reagent Strip Analysis
    • Semi-Automated Instruments
    • Automated Systems
    • Quality Control
    • Semi-automated and automated urine reagent strip readers offer what advantage over manual methods?
    • Urine chemical reagent strips readers can utilize any manufacturers dipsticks.
    • When an automated or semi-automated method is used to read urine chemical reagent strips, quality control testing must be performed at least how often...
• References
    • References