Extracellular Vesicles: From Biology to Biomedical Applications 2020

Extracellular Vesicles: From Biology to Biomedical Applications is organized by European Molecular Biology Laboratory (EMBL) and will be held from Nov 22 - 27, 2020 at EMBL Heidelberg, Heidelberg, Baden-Wurttemberg, Germany. 

Audience:
This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.

Course Overview:
A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterisation techniques and will address strategies to understand their role in biomedical applications.

Learning Outcomes:
In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.

Modules/Resources:
• Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques, size-exclusion chromatography (SEC))
• Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, nanoparticle tracking analysis, in vivo imaging, proteomic and transcriptomic analysis of EV)
• Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
• Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)

Additional details will be posted as soon as information is available.