Pinaki Banerjee PhD

Dr. Pinaki Prosad Banerjee has a Ph.D. in adaptive immunity, where he defined CD4T cell activation by a costimulatory molecule, which was derived from B cells. During his Ph.D, he also worked on B cell-mediated CD8T cell and macrophage-mediated CD4 T cell activation. His postdoctoral study focused on innate immunity, where he worked on PBMC-derived NK cells with special emphasis on Primary Immunodeficiencies and the changes of NK cell surface markers in health and disease conditions.

At present, Dr. Banerjee’s interest is to study changes in immune markers on therapeutic cells before and after these cells are infused into the patients, and also during the treatment. He is further interested to learn how the immune cells from the bodies of the patients change over time and relating these changes to the response to the therapy. For these in-depth immune-correlative studies, he implies most of the aspects of flow cytometry. In brief, Dr. Banerjee designs and sets up 15 + color panels on conventional flow cytometers to get an overall idea of the immune markers.

Side by side, using a Spectral flow-cytometer, he also establishes 30+ color panels and analyzes the distribution of the expression of the markers on single cell basis. Further to see the localization of the markers in and/or on the surface of the cells, Dr. Banerjee uses the imaging flow cytometer and develops algorithms to analyze the data. He is also interested in single-cell genomics and has established a method in the laboratory to study the immune cells on a single-cell level by a combination of flow cytometry and molecular biology-based methods. Moreover, at present, he is working on RNA sequencing on a single-cell level.