Nicola Zamboni graduated in the group of Jay Bailey at the Institute of Biotechnology of ETH Zurich, where he also received his Ph.D. degree in 2003 in the field of metabolic engineering and 13C metabolic flux analysis. In 2004, he moved as a PostDoc to the Stanford Genome Technology Center to deepen his knowledge on mass spectrometry and to apply metabolomics-based approaches for unraveling causes of metabolic changes in eukaryotic cells. This activity continues currently at ETH Zurich, where he returned in 2005 as a Principal Investigator in the Institute of Molecular Systems Biology of ETH Zurich 2005. The lab focuses on the development of experimental methods to characterize the in vivo response of complex metabolic networks to perturbations and environmental cues and reverse engineer cellular regulation.