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Home  >  Speakers  >  Florian Hollfelder
Florian Hollfelder

Florian Hollfelder

    
Cambridge, England    United Kingdom
Biochemistry And Molecular Genetics, Biotechnology,...More
Biochemistry, Catalysis,...More
Spoken at 1 event

Biography :

Publications

• Fischlechner M, Schaerli Y, Mohamed MF, Patil S, Abell C and Hollfelder F (2014) Microfluidic Gel-Shell Beads: Evolution of Catalysts Caged in Biomimetic Compartments. Nature Chemistry 2014, 6(9):791-6.

• van Loo, B.; Jonas, S.; Babtie, A. C.; Benjdia, A.; Berteau, O.; Hyvönen, M.; Hollfelder, F., An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proc. Natl Acad. Sci USA 2010, 107 (7), 2740-5.

• Villiers, B. R.; Hollfelder, F., Narrowing the Size Selectivity of an A-domain by Directed Evolution. Chemistry & Biology 2011,18(10):1290-9. • Kintses, B.; Hein, C.; Mohamed, M. F.; Fischlechner, M.; Courtois, F.; Lainé, C.; Hollfelder, F. Chemistry & Biology 2012, Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution, 19(8):1001-9.

• Diamante, L.; Gatti-Lafranconi, P.; Schaerli, Y.; Hollfelder, F., In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel 2013, 26(10):713-24.

• Mohamed, M.F. & Hollfelder, F. Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer. Biochim Biophys Acta 2013, 1834, 417-2.

• Kwok, A., Eggimann, G.A., Reymond, J.L., Darbre, T. & Hollfelder, F. Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure-activity relationships highlight the role of charge distribution across dendrimer generations. ACS Nano 2013, 7, 4668-82.

• Gielen, F.; van Vliet, L.; Koprowski, B. T.; Devenish, S. R.; Fischlechner, M.; Edel, J. B.; Niu, X.; Demello, A. J.; Hollfelder, F., A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition. Anal Chem 2013, 85(9):4761-9.

• Golicnik, M.; Olguin, L. F.; Feng, G.; Baxter, N. J.; Waltho, J. P.; Williams, N. H.; Hollfelder, F., Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. J Am Chem Soc 2009, 131 (4), 1575-88.

• Shim, J. U.; Olguin, L. F.; Whyte, G.; Scott, D.; Babtie, A.; Abell, C.; Huck, W. T.; Hollfelder, F., Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. J Am Chem Soc 2009, 131 (42), 15251-6.

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Specialties

Biochemistry and Molecular Genetics, Biotechnology, Research/Clinical Research

Interested Topics

Biochemistry, Catalysis, Chemical Biology, Organic Synthesis, Biophysical analysis, Microengineering, Mircrodroplets

State Licenses

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Board Certifications

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Professional Association

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Languages

English

Conference Session Materials

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