HIV: Structure, Replication, and Detection

HIV: Structure, Replication, and Detection is organized by MediaLab, Inc.

Description:
This introductory-level course is designed to meet requirements for HIV/AIDS training, as well as provide basic information about structure and the mechanisms and proteins involved in replication. Appropriate for continuing education for all laboratory personnel. Dr. Pendergraph's extensive experience teaching in the laboratory environment ensures that you get the training you need for your safety and your requirements.

Objectives:
• Explain the basic structure of HIV.
• Discuss the process by which HIV replicates.
• Discuss commonly used laboratory methods for detection of HIV infection.
• Compare the advantages and disadvantages of commonly used laboratory test methods for detection of HIV infection.

Course Outline:
• Structure of the Human Immunodeficiency Virus
    • Basic Structural Components
    • HIV Envelope
    • HIV Capsid
    • Viral proteins are identified as either glycoprotein (gp) or protein (p). What is indicated by the number that follows the "gp" or "p" designation, fo...
• Replication
    • Proteins Involved in Adsorption
    • Adsorption
    • Adsorption, continued
    • Penetration and Infection
    • DNA Conversion from RNA
    • HIV is a Retrovirus
    • Infection of the Host Cell
    • Infection of the Host Cell, continued
    • Spread of Infection
    • Spread of Infection, continued
    • Spread of Infection, continued
    • Destruction of the Host Cell
    • What is the protein that coats the genome in the core of the HIV-1 virion?
    • The enzyme found in the core of the HIV virion that is utilized to convert viral RNA into DNA is called reverse transcriptase.
• HIV Virus Classifications
    • Mutations
    • Most HIV-1 infections are classified as which one of the following groups?
• Progression of HIV Infection
    • Stages of HIV Infection
    • Which stage of HIV infection is characterized by a decline in the CD4 T-lymphocyte count to less than 200 cells/µL?
• Laboratory Methods to Detect and Monitor HIV Infection
    • HIV Test Methods: Introduction
    • Anti-HIV Immunoassays
    • HIV Immunoassay Methods
    • Rapid Anti-HIV Assays Using Alternate Specimen Types
    • Western Blot
    • Nucleic Acid Testing (NAT)
    • Reverse Transcriptase PCR (RT-PCR)
    • HIV Detection Methods: Comparison Table
    • The Centers for Disease Control and Prevention (CDC) Recommended Algorithm for HIV Testing
    • Which of the following HIV tests would be the BEST CHOICE to detect HIV infection in a high-risk seronegative individual?
• References
    • References