Body Fluid Differential Tutorial

Body Fluid Differential Tutorial is organized by MediaLab, Inc.

Course Overview:
If you have forgotten the art of differentiating choroid plexus cells from ependymal cells or viral lymphocytes from fluid monocytes, you are in luck! This course provides a thorough morphologic identification of over 40 cell types and disease states to recharge your memory. The course features over 100 crystal clear, perfectly stained body fluid images to illustrate both basic and advanced cellular morphology - perfect for a comprehensive, detailed review which can help refresh any technologist, both in or out of the hematology laboratory. The course is P.A.C.E. approved for 2 full credit hours to help reach your continuing education credit requirements. Enjoy learning and/or reviewing the key identification characteristics of body fluid cells in a user-friendly and fun format. Enroll today!

Objectives:
• Compare and contrast the morphology of cells found in normal cerebrospinal fluid (CSF), normal pleural fluid, normal peritoneal fluid, and normal synovial fluid.
• Identify the morphology of cells found in bronchoalveolar lavage (BAL).
• Distinguish abnormal/reactive/infected cell morphology in fluids.
• Recognize malignant cell morphology in body fluids.
• Discuss appropriate scenarios for hematology/pathology review.

Course Outline:
• Cytocentrifugation Morphology
    • Cytocentrifugation of Body Fluid Samples
• Cell Types Common To Most Types of Body Fluids
  • Neutrophils
    • Neutrophils
    • Neutrophils, continued
    • Eosinophils vs. Neutrophils
    • Nucleated blood cells will always have the same appearance on a cytocentrifuged smear as they do on a peripheral blood smear.
  • Lymphocytes, Atypical Lymphs, Plasma Cells
    • Normal Lymphocytes
    • Lymphocytes
    • Lymphocytes vs. Monocytes
    • Atypical Lymphocytes vs. Monocytes
    • Lymphocytes and Atypical Lymphocytes
    • Atypical Lymphocytes
    • Viral Lymphocytes
    • Lymphocytes and Plasma Cells in Body Fluids
    • The image on the right is a representative field from a cytospin preparation of a CSF from a patient with a viral infection. Identify the cell indicat...
  • Monocytes and Macrophages
    • Monocytes
    • Monocytes vs Lymphocytes
    • Monocytes and Macrophages
    • Macrophages
    • Phagocytosis
    • Hemosiderin
    • Histiocytes
    • (True/False) Macrophages are the phagocytes that originate from lymphocytes.
    • Match each cell type listed in the drop down box to the letter that corresponds to its identification in the image on the right.
• Cells Found in Cerebrospinal Fluid
  • Cerebrospinal Fluid: Non-Blood Cells
    • Ependymal Cells
    • Ependymal Clumps
    • Choroid Plexus Cells
  • Cerebrospinal Fluid: Bone Marrow Contamination
    • Bone Marrow Contamination in CSF
    • What is the identification of this cellular clump found in CSF? Note the presence of many similar-appearing nuclei without distinct lines of demarcati...
• Cells Found in Pleural and Peritoneal Fluids
  • Mesothelial cells
    • Mesothelial Cells
    • Mesothelial Cells, continued
    • Reactive Mesothelial Cells
• Cells Found in Synovial Fluids
    • Synovial Lining Cells
    • Synovial Lining Cells, continued
• Cells Found in Bronchoalveolar Lavages
  • Bronchial Lining Cells
    • Bronchial Lining Cells
    • Bronchial Lining Cells, continued
    • Match the following cell types to the body fluids in which they may be seen when the fluid is observed on a cytocentrifuged preparation.
• Infectious Organisms in Body Fluids
    • Infectious Organisms in Body Fluids
  • Bacteria
    • Intracellular and Extracellular Bacteria
    • Intracellular Bacteria
    • Peritoneal Lavage Trauma
    • Trauma STAT
    • Septic Arthritis
  • Fungus
    • Yeast
    • Candida albicans - Germ Tube Formation
    • Hyphae
  • Toxoplasma
    • Central Nervous System (CNS) Toxoplasmosis
    • Central Nervous System (CNS) Toxoplasmosis, continued
    • The elements indicated by the arrows were observed on a Wright-stained smear of a synovial fluid. What is the likely identification of the elements an...
• Malignant Fluids with Leukemia and Lymphoma.
    • Leukemic Fluids
  • Lymphoid Leukemia / Lymphomas
    • Acute Lymphoblastic Leukemia (ALL): L1 Morphology
    • Central Nervous System (CNS) Relapse: L2 Acute Lymphoblastic Leukemia (ALL)
    • Burkitt Lymphoma
    • Non-Hodgkin Lymphoma
    • Anaplastic Large Cell Lymphoma (ALCL)
  • Myeloid Leukemias
    • Acute Myeloid Leukemia (AML)
    • Acute Myeloid Leukemia (AML), continued
    • Acute Monoblastic Leukemia (AMoL)
    • Prominent vacuolation involving the cytoplasm of abnormal lymphoblast-like cells seen in a body fluid preparation is a distinctive feature of Burkitt ...
• Malignant Fluids with Metastatic Tumor
    • Metastatic Tumors in Fluid Cytospins
    • Adenocarcinoma in Peritoneal Fluid
    • Alveolar Rhabdomyosarcoma (ARMS) in Pleural Fluid
    • Atypical Teratoid/Rhabdoid Tumors (ATRT) in Cerebrospinal Fluid
    • Metastatic Melanoma in the Cerebrospinal Fluid (CSF)
    • Primitive Neuroectodermal Tumor (PNET) in Cerebrospinal Fluid
    • Medulloblastoma in Cerebrospinal Fluid
    •  Neuroblastoma in Pleural Fluid
    • Neuroblastoma Tumor Clump vs. Mesothelial Cells in Pleural Fluid
    • Retinoblastoma in Cerebrospinal Fluid
    • Match the morphologic characteristics with the listed cell type.
• References
    • References