Tissue Engineering Course: From Stem Cells to Organoids

Tissue Engineering Course: From Stem Cells to Organoids is organized by Centre for Genomic Regulation (CRG) and would be held during Jul 28 - Aug 04, 2017 at Centre for Genomic Regulation (CRG), Barcelona, Cataluna, Spain.

Selected participants will get an overview of the background and current methods for the generation and characterization of pluripotent stem cells, primordial germ cells, organoids and their genetic manipulation by CRISPR/Cas9 technology. They will have the opportunity to learn and practice how to generate and characterize.

• Mouse embryonic stem cells and induced pluripotent stem cells by means of morula aggregation and teratoma assays
• Primordial germ cells
• Cerebral, liver and intestinal organoids
• Genetically modified organoids by CRISPR/Cas9 technology

Organoids are revolutionizing the fields of developmental and stem cell biology as they have enabled the in vitro generation of complex structures resembling whole organs. They can be generated either from resident progenitors in adult organs or from pluripotent stem cells (ES and iPSc). When stem cells are grown in a 3D environment, they recapitulate the mechanisms of organogenesis seen in vivo. They are able to differentiate, go through the processes of cell-sorting and acquire the specific tissue patterning to develop into several endoderm- mesodem and ectoderm-derived tissues, mimicking their in vivo counterparts. These complex structures provide a unique opportunity to model human organ development in a system remarkably similar to development in vivo.